Put on gloves and separate the larvae into several different mass sizes: 25, 50, 100, 200, 400. Day 2: These eggs have and larvae come out. This website may contain names, images and voices of deceased Aboriginal and TorresStrait Islanderpeoples. 2023 Jan 28;9(2):e13281. 0 If you aren't squeamish, and you think you might be interested in doing a project related to forensic entomology, then keep on reading. Garbage, compost, animal feces, spoiled meat, any rotting materials, etc. Body farms are research facilities where scientists study the decomposition of human remains in different types of settings. Cookie used to remember the user's Disqus login credentials across websites that use Disqus. Secure .gov websites use HTTPS Another challenge is where to obtain corpses. If so, why do you think that is? 35%: The student must provide collection techniques, including the process of sample collection and further information a forensic entomologist must gather during an . Third-instar larvae grow to between 15 mm and 20 mm before wandering off as pre-pupae. The cookie is used to store and identify a users' unique session ID for the purpose of managing user session on the website. Did the maggots in some cups appear to grow faster than maggots in other cups? Figure 2. 2006 Apr 30;137(3-4):333-44 The ADH is a constant for each stage.). The dissection consists of three parts: an external examination, the internal organs, and the mouth and gills. Eggs are laid typically during the winter. Illustration by Ari Tiziani Description: Victim found in the woods, covered with plant scraps to hide the body. hbbd```b``Z"@$K The sp_t cookie is set by Spotify to implement audio content from Spotify on the website and also registers information on user interaction related to the audio content. SiteLock sets this cookie to provide cloud-based website security services. t%J,c&\nV/&Hh1H HS"b\H/UQeVUZ0Tsh6\oI#xX{{4 If you recreated more than one crime scene, compare your observations of different crime scene cups. They stay cocooned in the body for about two weeks, this is because the temperature is a constant temperature of about 70 degrees. the species of insects that arrive at/leave the body as it goes through decomposition what factors most influence insect growth and survival? -, Med Vet Entomol. The individuals benefit from shared secretions which aid in digestion and produce an alkaline environment conducive to larval growth. Larvae develop through a second and third stage before pupating and then later emerging as adults. Plants don't like flies and other bugs any more than you do, and the natural oils they produce to protect themselves are amazingly effective at killing and repelling bugs of all sorts. Adult blow fliesfeed on a wide range of substances, including garbage, rotting vegetation, and other decaying materials, but the larvae of most blow fly species are scavengers that feed off of decaying animal carcasses or feces. If you are seeing blow fly activity indoors, chances are there is something rotting nearby. 1. Shes a proponent of labs, field trips, and hands-on learning in lieu of standard lectures, and encourages her students to make connections between science and matters of social and environmental justice whenever possible. This website uses cookies to improve your experience. Effect of temperature on development of blue bottle blow flies. A female blowfly lays up to 300 eggs at one time, and with numerous females visiting a corpse, the number of maggots can be immense. The full blow fly life cycle can take two to three weeks from egg to adult to complete, and most adults live from two weeks to a month. Using the La Brea Tar Pits as inspiration, look at the conditions that affect fossil formation. One challenge to setting up a body farm is finding secluded fields away from densely populated areas so that the bodies wont be disturbed. The research in this article was funded by National Institute of Justice award 2016-DN-BX-0204 awarded to Aaron Tarone, Texas A&M University, College Station, TX. It takes 23 hours for an egg to hatch into a first instar if the temperature is 70oF. Bookshelf Yang YQ, Lyu Z, Li XB, Li K, Yao L, Wan LH. In the process, their activities result in the decomposition of the body and the recycling of nutrients. For each measurement of maggot mass temperature, take several readings at different places within the mass. The life-cycle of a blowfly takes about 3-5 weeks and there are approximately 3 to 4 generations of blowfly annually. Maggots (fly larvae) are remarkable eating machines. Adult blowflies are attracted by areas of wool which are wet or contaminated with faeces, where they lay their eggs. Remember that it will take about 1 to2 weeks for the adult flies to emerge from the pupae, and then two or three days more for the adults to mate and lay larvae. Keep your sink free of dirty dishes, and keep countertops wiped down and clean. The flesh flies (scientific name Sarcophaga bullata) you will use in this project are an example of this type of insect. Write to us at We want to hear your tips and tricks! The larva stage is the most readily recognized and when all the food consumption is accomplished. Under the arm (Armpit) temperature: 99 F (37.2 C) or higher. Used to help protect the website against Cross-Site Request Forgery attacks. Life cycle of the black blow fly. Was your prediction supported by the data you collected? Because flies rapidly discover a body and their development times are predictable under particular environmental conditions, the time of death can be calculated by counting back the days from the state of development of flies living on the corpse. Effect of chilling on pupal developmental arrest and subsequent impact on quality control parameters of adult blowfly, The Growth Model of Forensically Important. NID cookie, set by Google, is used for advertising purposes; to limit the number of times the user sees an ad, to mute unwanted ads, and to measure the effectiveness of ads. A load balancing cookie set to ensure requests by a client are sent to the same origin server. What kinds of insects typically show up at a crime scene? Would you like email updates of new search results? This cookie is set by GDPR Cookie Consent plugin. After testing flies during their four larval stages and as adults, the researchers established a range of high and low temperatures for each group in which they experienced knockdown, defined as the inability to walk, or locomote. %%EOF Adult female blow flies arrive within minutes to lay eggs on a cadaver. This cookie is used to detect and defend when a client attempt to replay a cookie.This cookie manages the interaction with online bots and takes the appropriate actions. Description: Victim found in the woods, covered with a blanket to hide the body. Federal government websites often end in .gov or .mil. The pattern element in the name contains the unique identity number of the account or website it relates to. Come and explore what our researchers, curators and education programs have to offer. Body Farm Data and Observation Sheet (PDF), Body Farm Data and Observation Sheet (DOC). However, this is a. Once a female blow fly lays eggs in an animal carcass (she must consume protein first), wound, eyes, ears or nose, the first-stage larvae can hatch within 24 to 48 hours. Adult flies will emerge in 1 to 2 weeks. These cookies ensure basic functionalities and security features of the website, anonymously. This website uses cookies to ensure you get the best experience onourwebsite. Research the use of the blowfly in determining postmortem interval. But if you are doing most or all 14 crime scenes, you can use one control cup per several crime scenes. BMC Genomics. The minimal duration of development from oviposition to adult emergence was inversely related to temperature, ranging from 9.190.3 days at 35 C to 37.782.96 days at 15 C. . These cookies do not allow the tracking of navigation on other websites and the data collected is not combined or shared with third parties. It makes a difference for body temperature regulation (thermoregulation)." Dress for success: In warm weather, wear light, cotton . Before Depending on the environmental conditions of the area, insects might have a difficult time finding the corpses in your body farm. Epub 2015 May 14. Be sure to wear latex or nitrile gloves. MeSH Life cycle Emergence of the blowfly after winter is controlled by temperature. Life cycle stages for the Green Bottle Blow Fly is constant because all of them follows a systematic life cycle stages. Their front ends are armed with mouth hooks with which they rake in decaying flesh, shredded from the corpse. This cookie is set by GDPR Cookie Consent plugin. Explore seven different animal and plant habitats: coasts, freshwater, Antarctica, forests, woodlands, arid zone and urban. Figure 2. These flies can be dangerous to animals, however, if they are able to fester in and lay eggs in a wound or sore an animal may be carrying. hours, and then molt into third-stage maggots. Each adult female fly can lay approximately 200 eggs every few days throughout its life. Clipboard, Search History, and several other advanced features are temporarily unavailable. How precise do you think your calculations are? hW[kJ+}iaA)8I}qd$M}gVNO`E In other species, there is a simple changedevelopment of wings in grasshoppers, for example. For example, if your calculation corresponds to the ADH required to reach the 2nd instar, did you observe medium-size maggots? You have reached the end of the page. scibuddy@sciencebuddies.org. As young insects grow and develop, their bodies change in a process called metamorphosis. The area is shady, and the body is not exposed to the sun. Because arthropod development is linked directly to temperature, she also gathers daily temperature data from the nearest available weather station. Such measurements could provide evidence that a body has been transported after death and could provide an alternative explanation as to why some dead bodies are found uncolonized.. Eggs are laid typically during the winter. If your pet is harboring an open wound, he or she is vulnerable to having a wandering female blow fly lay eggs on them. We pay our respect to Aboriginal Elders and recognise their continuous connection to Country. temperatures, moisture, food, oxygen what might prevent insects from growing/surviving? These pests come inside looking for food sources like rotting produce or decaying meat. Issued by Microsoft's ASP.NET Application, this cookie stores session data during a user's website visit. 2022 Mar 24;13(4):319. doi: 10.3390/insects13040319. Decomposition of a corpse is a continual process that can take from weeks to years, depending on the environment. They are: ZH Day 1 is one day after you placed the cups outside. (They also have anterior spiracles). Blow flies are known to have a loud, distinct buzz, but they do not bite humans. Science Buddies Staff. Pupae are inactive, and do not feed. On hatching, first-instar larvae are roughly 2 mm long, growing to about 5 mm before shedding their skin. The egg stage is followed by the larval stage, then the pupa, and finally the adult emerges. Before you start the experiment, you should decide where your body farm will be located. Use resistance training as training for your everyday life. (Hall and Brandt, 2006) The mass of maggots can also cause a local increase in temperature. National Institute of Justice, "The Effects of Temperature on Blowfly Colonization of Decomposing Human Bodies," January 11, 2022, nij.ojp.gov: Research for the Real World: NIJ Seminar Series, Investigating upper thermal limits of forensically important blow flies to improve testimony in forensic entomology, Investigating Upper Thermal Limits of Forensically Important Blow Flies to Improve Testimony in Forensic Entomology. If you have any comments (positive or negative) related to purchases you've made for science projects from recommendations on our site, please let us know. *Whether or not you need this item depends on the crime scene scenario(s) you choose. present in clumps of up to 300; laying to hatching takes 1 day; Larva . Upon the animals death the larvae will continue to consume the carcass. Flies lay eggs and larvae in clumps, and the developing maggots mass together as they grow. The second instar larvae grow to around 10 mm before they shed their skins to become third-instar larvae. Z9Zp%)E{VA9'#g- The predatory maggots of Chrysomya rufifacies are covered with spiny protrusions which deter other predators. The blowfly Lucilia cuprina is a primary colonizer of decaying vertebrate carrion, and its development provides a temperature-dependent clock that may be used to estimate the post-mortem interval . Google DoubleClick IDE cookies are used to store information about how the user uses the website to present them with relevant ads and according to the user profile. Bethesda, MD 20894, Web Policies The Effects of Temperature on Blowfly Colonization of Decomposing Human Bodies Development of blowflies on a body is a standard forensic measure of time since death, but temperature variations can alter that development and mislead investigators. Pieces of meat or liver will serve as stand-ins for corpses. AgriLife Extension's online Bookstore offers educational information and resources related to our many areas of expertise and programming; from agriculture, horticulture, and natural resources to nutrition, wellness for families and youth, and much more. Their digestive activities are so intense that the corpse heats up in the vicinity of a maggot mass, sometimes reaching 53 celsius. On the final day of observation, use forceps or plastic utensils to unwrap any meat wrapped in fabric or cloth. Heat waves can be dangerous, causing illnesses such as heat cramps and heat stroke, or even death. What factors could have influenced when the eggs were laid in the cup. which can raise the temperature around them more than 10 C. After more feeding, the third-stage maggots move away from the body and metamorphize into . The male is sexually mature at emergence. Blow flies typically breed outside and then make their way into homes. Do not wrap the plastic around the meat. fresh- begins at death, flies begin to arrive, temperature falls, autolysis occurrs bloat- swells due to gases produced by bacteria, temperature rise of the corpse, decay- gases subside, decomposition fluids seep from body, bacteria and maggots break through the skin., post-decay- carcass reduced to hair, skin, and bones Leave us a comment! The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Thank you for the information on the Blow Fly. This cookie is set by GDPR Cookie Consent plugin. The area is shady, and the body is not exposed to the sun. These cookies help provide anonymized information on metrics the number of visitors, bounce rate, traffic source, etc. As with most insects, the blow fly goes through a full metamorphosis (egg, larva, pupa, adult), though this particular fly species develops through three larval stages. Epub 2016 Sep 17. Essarras A, Pazzi M, Dadour IR, Magni PA. Sci Justice. Set by the GDPR Cookie Consent plugin, this cookie is used to record the user consent for the cookies in the "Advertisement" category . Copyright 2002-2023 Science Buddies. The student must provide a detailed account of the life cycle of the blowfly, including the life stages, length of the life cycle, and factors that affect this length. Download figure Open in new tab Hua can describe both fruit and egg. In research supported by the National Institute of Justice, researchers from Texas A&M University noted that although blowfly development in cadavers seems accurate and precise within moderate temperature ranges it remains largely untested against more extreme temperatures observed on decomposing carcasses. Principle investigator Aaron Tarone, an entomologist, said in a report that his teams research provides information as to how suboptimal environmental conditions, such as heat waves or cold snaps, affect adult colonization and larval development. The research, he wrote, attempts to better understand basic aspects of blowfly thermal biology in order to improve our use of blowflies as evidence in death investigations.. In this activity, you will create your own mini-body farm to attract insects to a corpse (in this case, a piece of meat). 1/3" - 2/5" in length. If forensic entomologists can determine the life stage of maggots on a corpse at a crime scene, they can estimate how long the body was there. Controversy in Forensic Entomology and Crime Scene Investigation: The Question that Is Bugging Forensic Entomologists, Decomposition: fly life cycle and development times. At these facilities, forensic scientists study how bodies decompose, and forensic entomologists study the insects that contribute to that decomposition. HWmo6_0S$xh +-bl*r8I;y{TjWvbggij{fe_Oi;3lc}lwCV5[|_do? hb``g``Z01G?3030332>e|"g"bP3_*i&upSX@l` 0 A How does temperature affect the life cycle of a blowfly? )i0&q y4DPH@\&Y 5Y$;AGxZ&'- SrG4Fhh#fP^wPToL;!3GlvFIUi;-CCJf+M r|. Maggots are gregarious animals and travel around in 'maggot masses'. eCollection 2023 Feb. Wang Y, Li L, Hu G, Kang C, Guo Y, Zhang Y, Wang Y, Wang J. Insects. Tip: Conduct this experiment when insects are most activethat is, when temperatures are above 60F during the daytime, and theres little to no rain. They thrive best in warm, humid weather. Each deposits about 250 eggs in the natural openings of the body and open wounds. Adult female blow flies arrive within minutes to lay eggs on a cadaver. df.`,"Y7eEl"x lZglP~ X (Kouyoumdjian, 2006) You'll want to think carefully about the best place to conduct your experiment. They vary depending on the species and the temperature. The insect-rearing box was kept in the artificial climate incubator LHP-300H (Yingmin Co. Ltd, Suzhou, China) at constant temperatures of 16C, 19C, 22C, 25C, 28C, 31C or 34C, with 75% humidity and photoperiod of 12 L : 12 D ( figure 2 ). The https:// ensures that you are connecting to the Once you receive the larvae, it could be as few as 9 or as many as 16 days until you have third instar larvae to study. 2020 Jan 29;21(1):92. doi: 10.1186/s12864-020-6509-0. Flesh flies generally arrive a day later. LockA locked padlock Reproduction of material from this website without written permission is strictly prohibited. Figure 3. To understand how, lets take another look at the Green Bottle Fly Life Cycle to see how long it takes a larva to develop at 70F into an adult. In a few species, for example silverfish, only size changes. Three levels of diversity work together to create the complexity of life on Earth. Description: Victim found in a field, covered with a layer of leaves to hide the body. An adult fly emerging from a pupa. Day 1: Adult flies arrive at body and immediately lay their eggs. To do this project, you should do research that enables you to Normal fevers between 100 and 104 F (37.8 - 40 C) are good for sick children. The student must provide collection techniques, including the process of sample collection and further information a forensic entomologist must gather during an investigation. Why does temperature matter? The larvae feed on wet, living, or dead flesh. Jennifer OBrien is a teacher and the Global Studies Program Director at the Watkinson School in Hartford, Connecticut, where she has taught science since 1998. Unauthorized use of these marks is strictly prohibited. A forensic scientist enters a crime scene and sees some flies, maggots, and a few beetles on and around a dead body. Do not wrap the fabric around the meat. All information these cookies collect is aggregated and therefore anonymous. At the end of the third instar stage, the maggots disperse, seeking a location in soil before pupation. In the lab, the scientist identifies each insect to species and determines their exact developmental stage. If you wrapped the meat in plastic or fabric, leave the meat wrapped and only observe insects outside the wrapping. The cookie is used to store the user consent for the cookies in the category "Performance". LW.GOv Shown are (clockwise, from lower left) adults, eggs, first instar larvae, second instar larvae, third instar larvae, pupae. Day 3-7: During these days the larvae just grow and grow. Put each mass in its own container, each with its own piece of liver. Compare your observations of the crime scene(s) and control cup(s). This photograph shows the stages of the fly life cycle. Description: Victim found in a field, covered with a blanket to hide the body. FACT. How could the patterns you observed be useful in a real crime scene analysis? Write to us at. You might have to briefly lift up the laundry baskets in order to see better. MYTH. 8600 Rockville Pike This article is based on the grantee report Investigating upper thermal limits of forensically important blow flies to improve testimony in forensic entomology, by Aaron Tarone. J Insect Physiol. In warm weather, conducive to fly growth, maggots can consume 60 per cent of a human body in less than a week. endstream endobj startxref i1ZC2wv[=mV7?(30$K\@~ [@)t]{*?saRTfYuiUON.8 ]]v>A SrG$)w^v"\ZX)or"m9jzp Azure sets this cookie for routing production traffic by specifying the production slot. Illustrations in this resource were created by Ari Tizianifor Science Friday. This cookie, set by YouTube, registers a unique ID to store data on what videos from YouTube the user has seen. The Great Barrier Reef is the world's largest system of coral reefs, mangrove and estuarine environments, and the Great Barrier Reef Marine Park covers an area of about 348,700. The area is sunny, with no trees to provide shade. -. That isnt always the case, he said, citing the case of a body found in July on a hot concrete sidewalk in Las Vegas. competition for food, predators, reproduction limitation, toxic wastes Day 2: These eggs have and larvae come out. A core assumption for estimating the postmortem interval, Tarone noted, is that blowflies immediately colonize a body after death. The applications are wide-ranging, but the most frequent is to determine the minimum time since death (minimum post-mortem interval, or PMI) in suspicious death investigations. Insect larvae pass through multiple stages of growth, called instars. Since this is the initial stage, the ADH = 1610. 2015 Aug;253:48-54. doi: 10.1016/j.forsciint.2015.05.006. But opting out of some of these cookies may have an effect on your browsing experience. [H%x?Uis7`f3xY4_; C&sZ6"aoX[,qxg/dv>Lp3O0L?JR(jrnd'g/TJRW^A0(k7M?+;Rs0+bu#ie uj=%}0)]0B Tr=`JeG!]jIID*^W@Yl'5l69EVU)~G7.RCLC98Pr@rGxXtY5 XHDX There are about a half-dozen in the United States and a few in Australia, with more planned in other countries. sharing sensitive information, make sure youre on a federal Once blow flies have made it indoors in the fall, theywill likely seek out-of-the-way areas to hide and overwinter. The area is sunny, with no trees to provide shade. The short lifespan of these flies is advantageous for demographic measurements while . Join us, volunteer and be a part of our journey of discovery! A lock ( A female blowfly lays up to 300 eggs at one time, and with numerous females visiting a corpse, the number of maggots can be immense. Adult female blow flies arrive within minutes to lay eggs on a cadaver. The maggots over-winter in the ground as larvae or pre-pupae. 1994 Jul;8(3):245-54 . Place the sunny cups together under an upside-down laundry basket to protect the cups from scavengers. Because the temperature outside is always changing during the day, scientists use the average daily temperature for this calculation. You may print and distribute up to 200 copies of this document annually, at no charge, for personal and classroom educational use. This cookie, set by Cloudflare, is used to support Cloudflare Bot Management. Thank you for reading. When you place your cup outside, dont forget to include the control. This cookie is native to PHP applications. Based on your calculations, in what stage of development should the maggots have been in on the last day of the experiment? Lets practice by starting with ADH calculations for the green bottle fly using the Accumulated Degree Hour Data Sheet and the sample calculations below. Science Buddies, a 501(c)(3) public charity, and keep our resources free for everyone. Adults emerge from the puparium in one to two weeks at room temperature (Figure2, below). %PDF-1.5 % Selecting the Right Product Type: Spray, Bait, Dust or Trap? The area is shady, and the body is not exposed to the sun. The rate of ageing is thermally sensitive in ectothermic invertebrates and we examined the effects of temperature on both demographic rates of ageing and on cellular senescence in the blowfly, Calliphora stygia. We use cookies and those of third party providers to deliver the best possible web experience and to compile statistics. The larvae will feed on the decaying liver. As shown in Figure1 (above), fly larvae pass through three instar stages (two molts). You need to allow time for ordering the pupae, and then for emergence of the adults and development of the larvae. YSC cookie is set by Youtube and is used to track the views of embedded videos on Youtube pages. Impact of Constant Versus Fluctuating Temperatures on the Development and Life History Parameters of, J Forensic Sci. Check out the What's On calendar of events, workshops and school holiday programs. In other insect species, such as flies, there is a complete metamorphosis from the larval stage to the adult body form. 2016 Dec;95:98-104. doi: 10.1016/j.jinsphys.2016.09.009. Disinfect containers with 5% bleach solution before disposal. The videos and images below contain graphic documentation of real life, rotting human and animal corpses that may be disturbing to a younger audience. Blow fly life cycle typically takes 3 4 weeks depending on the species. The life-cycle of a blowfly isn't very long, and it doesn't take long until they make their way to a dead/decomposing body. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. Instead, scientists collect the larvae and transfer them to temperature-controlled incubators in the lab. Each female deposits about 200 to 400 eggs near an open wound. Warmer temperatures can also lead to a chain reaction of other changes around the world. This website uses cookies to improve your experience while you navigate through the website. Blow fly larvae are known as maggots, and look like tiny, pale-white worms. This cookie is used for storing country code selected from country selector. LinkedIn sets the lidc cookie to facilitate data center selection. Blow fly larvae are known as maggots, and look like tiny, pale-white worms. -, Vet Parasitol. What effect do you think temperature has on insect development? Description: Blow fly life cycle typically takes 3 - 4 weeks depending on the species. This cookie is used for load balancing purposes. For each time point, calculate the average and standard deviation of the temperature measurements for each maggot mass. What kind of data is included in the graph? Disinfect the containers with a 5% solution of household bleach in water before disposal. High fevers may bring on seizures or confusion in children. This video shows the kind of research that can be done at Texas State Universitys Forensic Anthropology Research Facility, a body farm located in San Marcos, Texas. The adults will feed on the liver, mate, and lay larvae. What patterns do you notice in the life cycle diagram? LinkedIn sets this cookie to store performed actions on the website. The life-cycle of a blowfly takes about 3-5 weeks and there are approximately 3 to 4 generations of blowfly annually. The length of the gonotrophic cycle varies the vectorial capacity of a mosquito vector and therefore its exact estimation is important in epidemiological modelling. How does temperature and moisture affect the length of the blowfly's life cycle? and transmitted securely. You can use this as a reference when determining the life cycle stage of the maggot you collected in your body farm. Once youre done observing, return the cups to their location under the laundry baskets in the bodyfarm. Insects, and especially flies, are attracted to the scent given off by a decomposing body soon after death. Blow flies share many of the feeding and breeding habits of house flies. A5f@Ht,a")e5vp~L+p6=Tpa_ qyx~.iPz>B9mFw?j GD'.AhOM)$$U))0)K'_S FyJX;wQ}([P:F U Blow flies share many of the feeding and breeding habits of house flies. Inspect and repair screens on doors and windows to prevent flies from coming inside. proof that animal decomposed in the exact spot bf6 according to mann, what 6 factors determine the rate at which a body decays? The temperature is considered to be a variable because it changes depending on the manipulation of the researcher in the experiment. {k1`1X&dDk{vI+-B2MP If you choose to do just a few of the crime scene scenarios, use one control cup for each crime scene. The larvae burrow into the tissue, drop to the ground when mature, and pupate before emerging as adults. Unable to load your collection due to an error, Unable to load your delegates due to an error. The fundamental assumption is that the body has not been dead for longer than it took the insects to arrive at the corpse and develop. A gradual negative sloping graph shows that as temperature increases the days required for a fly to reach adulthood decreases exponentially. [The succession of sarcophagus beetles on carrion and its application in forensic medicine]. Description: Victim found in a field, covered with plastic to hide the body. 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